阳大海
生物工程专业:博士生导师/硕士生导师
生物学专业:博士生导师/硕士生导师
生物与医药专业(生物工程领域):工程博士生导师/工程硕士生导师
【个人简介】
阳大海,教授,博士生导师。suncitygroup太阳新城与美国密歇根大学Gabriel Nunez教授实验室联合培养博士,2015年毕业后留校任教,2021年入选国家优青,2022年主持获批国家重点研发计划(青年科学家)项目。现担任suncitygroup太阳集团教工第二党支部书记,生物反应器工程国家重点实验室、农业农村部水生动物疫病专业实验室、上海海洋动物疫苗工程技术研究中心固定成员,中国水产学会水产动物免疫学专业委员会、中国动物学会斑马鱼分会委员。曾获中国科协第二批 “青年人才托举工程”、上海市人事局“浦江人才”和上海市教委“晨光计划”等人才项目资助。
课题组聚焦分子免疫学方向取得了系列研究成果:(1)解析了宿主细胞焦亡激活关键信号通路,鉴定了胞内寄生菌与细胞焦亡互作的关键毒力因子,包括:内毒素、溶血素、分泌系统效应物等;(2)建立了“先天免疫记忆”(训练免疫)研究平台,阐明了其调控炎症性疾病发生分子机制,为靶向药物的筛选提供了活体评价模型;(3)形成了以单细胞测序技术为平台的系统免疫学评价手段,可应用于临床样本中药物处理导致细胞变化规律的更精细化定位,助力抗炎药物效力评估。迄今,已在Immunity,Nature Communications,Cell Reports,Fundamental Research,PLoS Pathogens,The Journal of Immunology(3篇)等领域内高水平期刊发表研究论文30余篇,并与国内外单位合作在Nature,Cell,Cell Host & Microbe等期刊发表研究论文10余篇。
【研究方向】
以模式动物小鼠、斑马鱼和水产经济养殖鱼种大菱鲆(多宝鱼)为对象,从比较免疫学角度,聚焦训练免疫这种新的先天免疫记忆应答模式开展相关科研工作:
1. 采用多组学(单细胞转录组、代谢组、表观遗传组)、基因编辑、反向遗传操作等技术手段,研究宿主训练免疫激活关键细胞类型及信号通路,探索其调控脓毒症器官损伤、感染性肠炎等疾病的分子机制。
2. 以训练免疫激活理论及其分子靶标为导向,开展新型疫苗、免疫增强剂、靶向药物的设计/筛选及其活性评价。
【科研项目情况】
1. 国家重点研发计划(海洋农业与淡水渔业科技创新专项),2022YFD2402000,基于训练免疫的鲆鲽鱼类遗传机制解析及育种应用探索,2022.11-2026.12,200万元,项目负责人;
2. 国家重点研发计划(深海和极地关键技术与装备专项),2022YFC2804300,基因/结构/活性三维导向的极地深海放线菌新型药源分子的高效挖掘,2023.01-2025.12,50万元,任务负责人;
3.国家自然科学基金优秀青年科学基金,32122090,水产免疫生物学,2022.01-2024.12,200万元,项目负责人;
4.上海市“科技创新行动计划”面上项目,20ZR1415500,鱼类焦亡信号调控中性粒细胞胞外陷阱功能研究,2020.02-2023.06,20万元,项目负责人;
5.中国科协第二批“青年人才托举工程”,2016QNRC001,病原感染与免疫,2017.01-2019.06,45万元,项目负责人;
6.国家自然科学基金青年项目,31602187,细菌类硫氧还蛋白调控MAPK信号通路及鱼类抗感染机制的研究,2017.01-2019.12,21万元,项目负责人;
7.上海市“晨光计划” (A类),16CG33,斑马鱼Caspase-A炎症小体激活介导的宿主免疫保护分子机制,2017.01-2019.12,6万元,项目负责人;
8.上海市“浦江人才”(D类),2016PJD020,细菌毒力因子“类硫氧还蛋白”抑制斑马鱼MAPK信号通路并调控细菌感染的机制研究,2016.01-2018.12,20万元,项目负责人。
【教学工作】
1. 本科生专业选修课《生物物理学》,32学时/年,2学分;
2. 研究生专业选修课《现代疫苗学技术与进展》,32学时/年,2学分。
【代表性论文】(按时间倒序,# 共同第一作者,* 通讯作者):
[1]Weijie Chen#, Jianchang Huang#, Wei Wang#, Ying Wang#,Hao Chen, Qiyao Wang, Yuanxing Zhang, Qin Liu*, Dahai Yang*, Multi-tissue scRNA-seq reveals immune cell landscape of turbot (Scophthalmus maximus), Fundamental Research 2022, 2(4): 550-561.
[2]Di Mu#, Jin Yang#, Yu Jiang#, Zhuang Wang, Weijie Chen, Jianchang Huang, Yuanxing Zhang, Qin Liu, Dahai Yang*, Single cell transcriptomic analysis reveals neutrophil as orchestrator during β-glucan-induced trained immunity in a teleost fish, The Journal of Immunology 2022, 209(4): 783-795.
[3]Jiatiao Jiang, Wenwen Wang, Fei Sun, Yuanxing Zhang, Qin Liu, Dahai Yang*, Bacterial infection reinforces host metabolic flux from arginine to spermine for NLRP3 inflammasome evasion, Cell Reports 2021, 34(10): 108832.
[4]Weijie Chen, Jingjing Zhao, Di Mu, Zhuang Wang, Qin Liu, Yuanxing Zhang, Dahai Yang*, Pyroptosis mediates neutrophil extracellular trap formation during bacterial infection in zebrafish, The Journal of Immunology 2021, 206(8): 1913-1922.
[5] Zhuang Wang, Zhaoyan Gu, Qing Hou, Weijie Chen, Di Mu, Yuanxing Zhang, Qin Liu, Zhihong Liu, Dahai Yang*, Zebrafish GSDMEb cleavage-gated pyroptosis drives septic acute kidney injury in vivo, The Journal of Immunology 2020, 204(7): 1929-1942.
[6] Ying Wen, Shouwen Chen, Zhiwei Jiang, Zhuang Wang, Jinchao Tan, Tianjian Hu, Qiyao Wang, Xiangshan Zhou, Yuanxing Zhang, Qin Liu, Dahai Yang*, Dysregulated haemolysin promotes bacterial outer membrane vesicles-induced pyroptotic-like cell death in zebrafish, Cellular Microbiology2019, 21(6): e13010.
[7] Dahai Yang#, Xiaohong Liu#, Wenting Xu, Zhaoyan Gu, Cuiting Yang, Lingzhi Zhang, Jinchao Tan, Xin Zheng, Zhuang Wang, Shu Quan, Yuanxing Zhang, Qin Liu*, The Edwardsiella piscicida thioredoxin-like protein inhibits ASK1-MAPKs signaling cascades to promote pathogenesis during infection, PLoS Pathogens2019, 15(7): e1007917.
[8]Dahai Yang, Xin Zheng, Shouwen Chen, Zhuang Wang, Wenting Xu, Jinchao Tan, Mingyu Hou, Wenhui Wang, Zhaoyan Gu, Qiyao Wang, Ruilin Zhang, Yuanxing Zhang, Qin Liu*, Sensing of cytosolic LPS through caspy2 pyrin domain mediates noncanonical inflammasome activation in zebrafish, Nature Communications2018, 9(1): 3052.
[9] Hideki Hara, Sergey S Seregin,DahaiYang, Koichi Fukase, Mathias Chamaillard, Emad S Alnemri, Naohiro Inohara, Grace Y Chen, Gabriel Núñez*, The NLRP6 inflammasome recognizes lipoteichoic acid and regulates gram-positive pathogen infection. Cell2018, 175(6): 1651-1664.e14
[10] Hao Chen, Dahai Yang,Fajun Han, Jinchao Tan, Lingzhi Zhang, Jingfan Xiao, Yuanxing Zhang, Qin Liu*, The bacterial T6SS effector EvpP prevents NLRP3 inflammasome activation by inhibiting the Ca2+-dependent MAPK-Jnk pathway, Cell Host & Microbe2017, 21(1): 47-58
[11] Yuan He, Melogy Y Zeng,DahaiYang, Benny Motro, Gabriel Núñez*, NEK7 is an essential mediator of NLRP3 activation downstream of potassium efflux, Nature2016, 530(7590): 354-357.
[12] Dahai Yang, Yuan He, Raul Muñoz-Planillo, Qin Liu*, Gabriel Núñez*, Caspase-11 requires the pannexin-1 channel and the purinergic P2X7 pore to mediate pyroptosis and endotoxic shock, Immunity2015, 43(5): 923-932.
联系方式:
E-mail:dahaiyang@ecust.edu.cn