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魏东芝
生物学专业:博士生导师/硕士生导师
生物工程专业:博士生导师
生物化工专业:硕士生导师
生物与医药专业(生物工程领域):博士生导师/硕士生导师
国家二级教授,鲁华生物技术研究所所长,suncitygroup太阳集团学术委员会主任,上海生物工程学会理事长。
全国优秀科技工作者,国家重点学科-生物化工学科带头人,上海市重点学科-生物工程学科带头人,教育部跨世纪优秀人才、上海市科技领军人才,上海市优秀学科带头人, 中科院“百人计划”入选者。教育部生物技术与生物工程专业教指委副主任,中国微生物学会酶工程专业委员会副主任,中国生物工程学会常务理事。享受国务院特殊津贴和上海市特殊津贴,中国科协“九大”代表。
2002-2022年先后5次主持国家“科技重大专项”及重点研发项目,负责完成国家863、973课题、自然科学基金、国家科技攻关、上海市重点项目等20余项重大重点项目。发表SCI收录论文300余篇(包括Angewandte Chemie、Metabolic Engineering、Green Chemistry、Applied and Environmental Micorbiology、 Chemical Communications等国际顶级学术期刊),他引1万多次,发明专利70余项。获得国家新药临床证书3项,实现产业化项目30余项。已培养研究生500余人。
获2015年国家技术发明二等奖,2010-2023年省部级技术发明或科技进步一等奖5项。
创建鲁华生物技术研究所及学术团队
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研究领域:
研究领域:
1. 生物催化工程:酶/生物催化剂进化、定制,生物催化过程工程研究。
2. 合成生物学:面向医药、农药、化工、食品、化妆品及材料等领域,创建细胞工厂并推动国际领先的产业化应用。
代表性论文
2022
Lin Yang, Kaiyue Zhang, Meng Xu, Youyu Xie, Xiangqi Meng, Hualei Wang*, Dongzhi Wei*. Mechanism-Guided Computational Design of ω-Transaminase by Reprograming of High-Energy-Barrier Steps. Angew. Chem. Int. 2022,(52): 202212555.
Kun Liu, Yunsen Zhang, Ke Liu, Yunqiu Zhao, Bei Gao, Xinyi Tao, Ming Zhao, Feng-Qing Wang*, Dongzhi Wei*. De novo design of a transcription factor for a progesterone. Biosensors & Bioelectronics. 2022, 203: 113897.
Min Jiang, Yong-Jun Liu, Guo-Song Liu, Wei Zhou, Ya-Ling Shen, Bei Gao*, Feng-Qing Wang*, Dong-Zhi Wei*.Sequential resolution of (S) and (R)-6-fluoro-chroman-2-carboxylic acid by two esterases in turn. Green Chemistry. 2022,24: 3235-3242.
Kun Liu, Feng-Qing Wang*, Ke Liu, Yunqiu Zhao, Bei Gao, Xinyi Tao, Dongzhi Wei*. Light-driven progesterone production by InP–(M. neoaurum) biohybrid system. Bioresources and Bioprocessing. 2022, 9: 9.
Xie D, Du L, Lin H, Su E, Shen Y, Xie J*,Dongzhi Wei*.In vitro-in silico screening strategy and mechanism of angiotensin I-converting enzyme inhibitory peptides from α-lactalbumin. Lebensmittel-Wissenschaft und-Technologie,2022,156: 112984.
Du L, Ro K-S, Zhang Y, Tang Y-J, Li Wan, Xie J*, Dongzhi Wei*.Effects of Lactiplantibacillus plantarum X7021 on physicochemical properties, purines, isoflavones and volatile compounds of fermented soymilk. Process Biochemistry. 2022, 113: 150-157.
Xie D, Shen Y, Su E, Du L, Xie J*, Dongzhi Wei*.The effects of angiotensin I-converting enzyme inhibitory peptide VGINYW and the hydrolysate of α-lactalbumin on blood pressure, oxidative stress and gut microbiota of spontaneously hypertensive rats. Food & Function. 2022, 13: 2743-2755
Zhao L, Zhu J, Xie J*, Dongzhi Wei*. Discovery of a novel acrylic acid formation pathway in Gluconobacter oxydans and its application in biosynthesis of acrylic acid from glycerol. Process Biochemistry. 2022, 118: 182-189
2021
Min Liu, Yang-Chen Lin, Jiao-Jiao Guo, Meng-Meng Du, Xinyi Tao, Bei Gao, Ming Zhao, Yushu Ma, Feng-Qing Wang*, Dongzhi Wei*. High-level production of sesquiterpene patchoulol in Saccharomyces cerevisiae. ACS Synthetic and Biology. 2021, 10: 158-172.
Xiangqi Meng, Lin Yang, Yan Liu, Huali Wang*, Yaling Shen, Dongzhi Wei*. dentification and Rational Engineering of a High Substrate-Tolerant Leucine Dehydrogenase Effective for the Synthesis of L-tert-Leucine. ChemCatChem. 2021,13(14): 3340-3349
Liuzhu Wang, Shiqing Diao, Yangyang Sun, Shuiqin Jiang, Yan Liu, Hualei Wang* , Dongzhi Wei*.Rational engineering of Acinetobacter tandoii glutamate dehydrogenase for asymmetric synthesis of L-homoalanine through biocatalytic cascades. Catalysis Science & Technology. 20211, 14: 208-4215
Youyu Xie, Feng Xu, Lin Yang, He Liu, Xiangyang Xu, Hualei Wang* Dongzhi Wei*.Engineering the large pocket of an (S)-selective transaminase for asymmetric synthesis of (S)-1-amino-1-phenylpropane. Catalysis Science & Technology. 2021, 11: 2461-2470.
2020
Liu, Guo-Song, Li, Tian, Zhou, Wei, Jiang, Min,T ao, Xin-Yi, Liu, Min, Zhao, Ming, Ren, Yu-Hong, Gao, Bei*, Wang, Feng-Qing*, Wei, Dong-Zhi*. The yeast peroxisome: A dynamic storage depot and subcellular factory for squalene over production. Metabolic Engineering. 2020, 57:151-161
Yang, ZY, Fu, HW ,Ye, WJ ,Xie, YY , Liu, QH, Wang, HL, Dongzhi Wei*.Efficient asymmetric synthesis of chiral alcohols using high 2-propanol tolerance alcohol dehydrogenase SmADH2 via an environmentally friendly TBCR system. Catalysis Science & Technology. 2020, 10: 70-78.
2019
Jiajia Zhang, Yumeng Chen, Chuan Wu, Pei Liu, X Wei Wang*, Dongzhi Wei*. The transcription factor ACE3 controls cellulase activities and lactose metabolism via two additional regulators in the fungus Trichoderma reesei. Journal of Biological Chemistry. 2019, 294:18435-18450
2018
Zhongwei Yang, Hongling Wang, Yuxiao Wang, Yuhong Ren*, Dongzhi Wei*. Manufacturing Multienzymatic Reactors In Vivo by Self-Assembly to Improve the Biosynthesis of Itaconic Acid in Escherichia coli. ACS Synthetic Biology. 7(5), 1244-1250.
Zhiqiang Yao, Shuiqin Jiang, Lujia Zhang, * Bei Gao, Xiao He, John Z. H. Zhang, Dongzhi Wei *. Crius: A novel fragment-based algorithm of de novo substrate prediction for enzymes. PROTEIN SCIENCE. 2018, 27(8): 1526-1534.
Hao-Hao Liu, Li-Qin Xu, Kang Yao, Liang-Bin Xiong, Xin-Yi Tao, Min Liu, Feng-Qing Wang*, Dong-Zhi Wei*. Engineered 3-ketosteroid 9α-hydroxylases in Mycobacterium neoaurum: an efficient platform for production of steroid drugs. Applied and Environmental Microbiology. 2018, 84(14): E02777-17.
Min Liu, Liang-Bin Xiong, Xinyi Tao, Qing-Hai Liu, Feng-Qing Wang*, Dong-Zhi Wei*. Metabolic adaptation of Mycobacterium neoaurum ATCC 25795 in the catabolism of sterols for producing important steroid intermediates. Journal of Agricultural and Food Chemistry. 2018, 66(45): 12141-12150.
Min Liu; Liang-Bin Xiong; Xinyi Tao; Qing-Hai Liu; Feng-Qing Wang*; Dong-Zhi Wei*. Integrated transcriptome and proteome studies reveal the underlying mechanisms for sterol catabolism and steroid production in Mycobacterium neoaurum. Journal of Agricultural and Food Chemistry. 2018, 66(34): 9147-9157.
Zhen-hai Li, Feng-Qing Wang*, Dong-Zhi Wei*. Self-cloning CRISPR/Cpf1 facilitated genome editing in Saccharomyces cerevisiae. Biorescources and Bioprocessing. 2018, 5(1):36.
Yumeng Chen, Yaling Shen, Wei Wang* and Dongzhi Wei*. Mn2+ modulates the expression of cellulase genes in Trichoderma reesei Rut.C30 via calcium signaling,Biotechnology for Biofuels. 2018,11(1):54.
Bei Gao, Shuiqin Jiang, Liuzhu Wang, Lujia Zhang, Dongzhi Wei*. Energy and conformation determine the enantioselectivity of enzyme Biochemical Engineering Journal. 2018, 129: 106-112
Lingmeng Li, Fengying Dong, Lin Lin, Dannong He, Wei Wei*, and Dongzhi Wei*. N-Terminal Domain Truncation and Domain Insertion-Based Engineering of a Novel Thermostable Type I Pullulanase from Geobacillus thermocatenulatus. Journal of Agricultural and Food Chemistry. 2018, 66(44): 10788-10798
Lingmeng Li, Fengying Dong, Lin Lin, Dannong He, Jingwen Chen, Wei Wei,* Dongzhi Wei*. Biochemical Characterization of a Novel Thermostable Type I Pullulanase Produced Recombinantly in Bacillus subtilis. Starch. 2018, 70, 1700179
Xie Jingli, Chen Xujun, Wu Junjie, Zhang Yanyan, Zhou Yan, Zhang Lujia, Tang Ya-Jie, Wei Dongzhi*. Antihypertensive effects, molecular docking study, and isothermal titration calorimetry assay of angiotensin I‑converting enzyme inhibitory peptides from Chlorella vulgaris. Journal of Agricultural and Food Chemistry. 2018, 66: 1359-1368
Jiawei Zhu, Jingli Xie, Liujing Wei, Jinping Lin, Li Zhao* , Dongzhi Wei*. Identification of the enzymes responsible for 3-hydroxypropionic acid formation and their use in improving 3-hydroxypropionic acid production in Gluconobacter oxydans DSM 2003. Bioresource Technology. 2018, 265,:328-333.
2017
Xinyi Tao, Ning Jia, Nenghui Cheng, Yuhong Ren, Xuni Cao, Min Liu, Dongzhi Wei*, Feng-Qing Wang*. Design and evaluation of a phospholipase D based drug delivery strategy of novel phosphatidyl-prodrug. Biomaterials. 2017, 131: 1-14.
Liang-Bin Xiong, Hao-Hao Liu, Li-Qin Xu, Wan-Ju Sun, Feng-Qing Wang*, Dong-Zhi Wei*. Improving the production of 22-hydroxy-23,24-bisnorchol-4-ene-3-one from sterols in Mycobacterium neoaurum by increasing cell permeability and modifying multiple. Microbial Cell Factories.
Ying Liu, Xin Gao, Dongzhi Wei*, Yuhong Ren*. Reversible Photocontrol of Lipase Activity by Incorporating a Photo switch into the Lid Domain .Chemphotochem. 2017, 89: 2799.
Zhongwei Yang, Xin Gao, Hui Xie, Fengqing Wang, Yuhong Ren* and Dongzhi Wei*. Enhanced Itaconic Acid Production by Self-Assembly of Two Biosynthetic Enzymes in Escherichia coli. Biotechnology and Bioengineering. 2017, 1 (9): 393-396.
Min Jiang, Lujia Zhang, Fengqing Wang, Jie Zhang, Guosong Liu, Bei Gao*, Dongzhi Wei*. Novel application of magnetic protein: Convenient one-step purification and immobilization of proteins. Scientific Reports. 2017, 7: 13329.
Shuiqin Jiang, Lujia Zhang,* Zhiqiang Yao, Bei Gao, Hualei Wang, Xiangzhao Mao, Dongzhi Wei*. Switching a nitrilase from Syechocystis sp PCC6803 to a nitrile hydratase by rationally regulating reaction pathways. Catalysis Science & Technology. 2017, 7 (5) : 1122-1128.
Guo Mingrong, Chen Xujun, Wu Junjie, Zhang Lujia, Huang Weixue, Yuan Ying, Fang Ming, Xie Jingli*, Wei Dongzhi*. Angiotensin I-converting enzyme inhibitory peptides from Sipuncula (Phascolosoma esculenta): Purification, identification, molecular docking and antihypertensive effects on spontaneously hypertensive rats. Process Biochemistry. 2017,63:84-95
Wei Wang⁎, Yumeng Chen, Dong-Zhi Wei⁎. Copper-mediated on-off control of gene expression in filamentous fungus Trichoderma reesei,Journal of Microbiological Methods. 2017,143:63-65.
近期代表性专利
序号 | 专利名称 | 权利人 | 有效状态 |
1 | 一种亮氨酸脱氢酶突变体及其编码基因、基因工程菌以及在制备L-叔亮氨酸中的应用 | suncitygroup太阳新城 | 有效 |
2 | 降血压肽和降血压蛋白质及其应用 | suncitygroup太阳新城 | 有效 |
3 | 发酵剂、臭豆腐卤液的直投式发酵方法及臭豆腐卤液 | suncitygroup太阳新城 | 有效 |
4 | 一种分枝杆菌基因工程菌及其在制备甾体化合物中的应用 | suncitygroup太阳新城 | 有效 |
5 | Mutant lacking the hydroxyacyl-coenzyme a dehydrogenase gene and use there | suncitygroup太阳新城 | 有效 |
6 | 一种植物乳杆菌X7022的应用. 黑蒜酵素及其制备方法与应用 | suncitygroup太阳新城 | 有效 |
7 | Genetically-engineered Mycobacterium strain and a use thereof in the preparation of steroidal compounds | suncitygroup太阳新城 | 有效 |
8 | Hydroxyacyl-coenzyme a dehydrogenase gene and acyl-coenzyme A thiolase gene and geneticallyengineered bacteria and use thereof | suncitygroup太阳新城 | 有效 |
9 | 一种酶固定化方法及其应用 | suncitygroup太阳新城 | 有效 |
10 | 一种功能性发酵豆乳及其制备方法与应用 | suncitygroup太阳新城 | 有效 |
11 | 一种植物乳杆菌X7022及其应用 | suncitygroup太阳新城 | 有效 |
12 | 重组免疫毒素Gigantoxin-4-4D5 scFv及其制备方法和用途 | suncitygroup太阳新城 | 有效 |
13 | ヒドロキシアシル-CoAデヒドロゲナーゼ遺伝子、アシル-CoAチオラーゼ遺伝子、及びその遺伝子工学的細菌及びその応(羟酰辅酶A脱氢酶基因、酰基辅酶A硫解酶基因、及其基因工程菌以及应用) | suncitygroup太阳新城 | 有效 |
14 | A Genetically-Engineered Mycobacterium Strain And A Use Thereof In The Preparation Of Steroidal Compounds. | suncitygroup太阳新城 | 有效 |
15 | 一种S型的ω-转氨酶ATA-W12及其基因和应用 | suncitygroup太阳新城 | 有效 |
16 | 一种醇脱氢酶LC3及其基因和应用 | suncitygroup太阳新城 | 有效 |
联系方式
上海市梅陇路130号311信箱 邮编:200237
电话: 021-64252078
手机(微信): 13601699452
E-mail: dzhwei@ecust.edu.cn