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林金萍
生物工程专业:博士生导师
生物学专业:硕士生导师
生物化工专业:硕士生导师
生物与医药专业(生物工程领域):硕士生导师
个人简介:
林金萍,工学博士,副研究员,博士生导师。现任中国轻工业生物催化与智能制造重点实验室副主任,生物反应器工程国家重点实验室固定人员。1997年和2000年分别于南昌大学和无锡轻工大学获学士和硕士学位,2006年毕业于suncitygroup太阳新城,获生物化工专业博士学位。
长期从事生物催化与酶工程、合成生物学等领域相关研究工作。主持和参与国家重点研发计划、国家自然科学基金、“973”课题、重大专项以及企业横向项目20项。发表学术论文60余篇。申请发明专利 17项,已授权10项。获得国家技术发明二等奖1项、上海市技术发明一等奖1项和上海市科技进步奖一等奖2项。获得国家新药临床批件2项、生产批文2项和新药证书1项,实现成果转让3项。2016年入选“上海市青年科技英才”。
主要研究方向:
1. 酶的催化机制与构效关系解析
2. 酶分子设计与定向进化
3. 人工生物(多酶)合成途径构建与体外合成生物学催化技术的研究
4. 结合生物催化与微生物合成生物学技术,高效合成手性药物、天然产物、食品添加剂及功能化学品等
部分论文列表(*通讯作者):
1. Baoqi Zhang, Han Du, Yanqiu Zheng, Jiale Sun, Yu Shen, Jinping Lin*, Dongzhi Wei. Design and engineering of whole-cell biocatalyst for efficient synthesis of (R)-citronellal. Microbial Biotechnology. 2022,15(5), 1486-1498
2. Xinlei Mao, Baoqi Zhang, Chenxiu Zhao, Jinping Lin*, Dongzhi Wei. Overexpression of mGDH in Gluconobacter oxydans to improve d‑xylonic acid production from corn stover hydrolysate. Microbial Cell Factories. 2022, 21:35
3. Baoqi Zhang, Jiale Sun, Yanqiu Zheng, Xinlei Mao, Jinping Lin *, Dongzhi Wei. Identification of a novel ene reductase from Pichia angusta with potential application in (R)-levodione production. RSC Advances. 2022, 12, 13924 – 13931
4. Yanqiu Zheng , Baoqi Zhang , Yuqinxin Xie , Jinping Lin *, Dongzhi Wei. Using a novel data-driven combinatorial mutagenesis strategy to engineer an alcohol dehydrogenase for efficient geraniol synthesis. Biochemical Engineering Journal. 2022, 186:108568
5. Wen Tang, Lulu Chen, Jian Deng, Yuyao Kuang, Chao Chen, Bo Yin, Hualei Wang,Jinping Lin*, Dongzhi Wei. Structure-guided evolution of carbonyl reductase for efficient biosynthesis of ethyl (R)-2-hydroxy-4-phenylbutyrate. Catalysis Science & Technology. 2020, 10: 7512
6. Yidi Liu, Xinlei Mao, Baoqi Zhang, Jinping Lin*, Dongzhi Wei. Modification of an engineered Escherichia coli by a combinatorial strategy to improve 3,4-dihydroxybutyric acid production. Biotechnology Letters, 2021, 43:2035–2043
7. Liandan Zheng, Jinping Lin*, Baoqi Zhang, Yuyao Kuang, Dongzhi Wei. Identification of a yeast old yellow enzyme for highly enantioselective reduction of citral isomers to (R)‑citronellal. Bioresources and Bioprocessing. 2018, 5:9
8. Xingxing Zou‡,Jinping Lin‡, Xinlei Mao, Shengyun Zhao,Yuhong Ren*. Biosynthesis of L‑erythrose by assembly of two key enzymes in Gluconobacter oxydans. Journal of Agricultural and Food Chemistry. 2017, 65 (35), 7721
9. Kangling Chen, Kefei Li, Jian Deng, Baoqi Zhang,Jinping Lin*, Dongzhi Wei. Carbonyl reductase identification and development of whole-cell biotransformation for highly efficient synthesis of (R)-[3,5-bis(trifluoromethyl)phenyl] ethanol. Microbial Cell Factories. 2016, 15(1):191
10. Huan Zhang, Lulu Shi, Xinlei Mao,Jinping Lin*, Dongzhi Wei. Enhancement of cell growth and glycolic acid production by overexpression of membrane-bound alcohol dehydrogenase in Gluconobacter oxydans DSM 2003. Journal of Biotechnology. 2016, 237: 18
11. Jian Deng, Kangling Chen, Zhiqiang Yao, Jinping Lin*, Dongzhi Wei*. Towards the computational design and engineering of enzymeenantioselectivity: A case study by a carbonyl reductase from Gluconobacter oxydans. Journal of biotechnology. 2016, 217: 31
12. Kefei Li, Xinlei Mao, Liu Liu,Jinping Lin*, Ming Sun, Dongzhi Wei and Shengli Yang. Overexpression of membrane‑bound gluconate‑2‑dehydrogenase to enhance the production of 2‑keto‑d‑gluconic acid by Gluconobacter oxydans. Microbial Cell Factories. 2016, 15(1):121
13. Yuanyuan Shi, Kefei Li, Jinping Lin*, Dongzhi Wei, Shengli Yang. Engineered expression vectors significantly enhanced the production of 2-keto-D-gluconic acid by Gluconobacter oxydans. Journal of Agricultural and Food Chemistry.2015, 63(22): 5492
14. Xingxing Zhang, Baoqi Zhang,Jinping Lin*, Dongzhi Wei. Oxidation of ethylene glycol to glycolaldehyde using a highly selective alcohol dehydrogenase from Gluconobacter oxydans. Journal of Molecular Catalysis B: Enzymatic. 2015, 112: 69
15. Rong Chen, Xu Liu, Jiale Wang,Jinping Lin*, Dongzhi Wei*. Cloning, expression, and characterization of an anti-Prelog stereospecific carbonyl reductase from Gluconobacter oxydans DSM2343. Enzyme and Microbial Technology. 2015, 70: 18
专著教材:
1.副主编:《酶工程》,高等教育出版社,2020. ISBN 978-7-04-054779-5
2.参编:《生物催化剂与酶工程》,科学技术出版社,2008.ISBN 978-7-03-022195-7
授权发明专利:
1.林金萍, 魏东芝,石圆圆。一种适用于氧化葡萄糖酸杆菌的基因表达载体及其应用。ZL 201510042193.1
2.林金萍, 魏东芝,张行星。一种醇脱氢酶氧化二元伯醇的方法。ZL 201310606518.5
3.林金萍, 魏东芝,张行星。一种应用微生物细胞催化法制备羟基醛的方法。 ZL 201310601537.9
4.林金萍, 魏东芝,李克非。一种微生物细胞转化法生产2-酮基-D-葡萄糖酸的方法。ZL 201310598049.7
5.林金萍, 魏东芝,施露露。一种氧化葡萄糖酸杆菌启动子及其应用。ZL 201310597667.X
6.林金萍, 魏东芝,杨圆圆。一种硝基还原酶在芳香族硝基化合物降解还原中国的应用。ZL 201310627211.3
7.林金萍,魏东芝,吴建。一种同时分析微生物发酵液中甘油和二羟基丙酮的方法。ZL 200910052444.9
8.林金萍, 魏东芝, 李明华, 吴建。一种氧化葡萄糖酸杆菌(G.oxydans)的基因工程菌及其应用。ZL 20091096972.1
9.魏东芝,林金萍,高琼。一种微生物不对称拆分制备(S)-苯乙二醇的方法。ZL 200810038899.0
10.魏东芝,林金萍,张敏洁。一种酶法连续生产聚(ε-己内酯)的方法。ZL 200910045661.5
获奖:
1.定向转化多元醇的生物催化剂创制及其应用关键技术。国家技术发明二等奖,2015
2.定向转化多元醇的细胞催化剂创制及其应用关键技术。上海市技术发明奖,一等奖,2013
3.手性非天然氨基酸生物催化制备技术及其应用。上海市科技进步奖,一等奖,2012
4.工业酶制剂的开发与应用关键技术。上海市科技进步奖,一等奖,2010
教学情况:
1. 本科生专业必修课《酶工程》(国家精品课程,国家级一流本科课程)、本科生专业选修课《食品生物技术》、《环境生物技术》
2. 研究生专业必修课《酶学与生物催化》,研究生专业选修课《结构生物学》
联系方式:
上海市梅陇路130号311信箱 邮编:200237
办公室:鲁华生物技术研究所(实验14楼东楼)402室
电话: 021-64251923;13918729416
E-mail: jplin@ecust.edu.cn